KEY RESOURCES TABLE.
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
| ||
Anti-ZBP1, mAb (Zippy-1) | Adipogen | Cat# AG-20B-0010-C100; RRID:AB_2892147 |
DYKDDDDK Tag (D6W5B) Rabbit mAb | Cell Signaling | Cat# 14793; RRID:AB_2572291 |
Monoclonal Anti-Vaccinia Virus E3L, Clone TW2.3 (produced in vitro), NR-4547 | BEI Resources, NIAID, NIH | Cat# NR-4547; RRID:AB_2892148 |
E3L Rabbit polyclonal Ab | Generated in Jacobs Lab | 10.1016/0042-6822(91)90768-7; RRID:AB_2892150 |
Anti-β-Actin (Clone AC-74 mAb) | Sigma | Cat# A5316; RRID:AB_476743 |
Anti-GAPDH (Clone G-9 mAb) | Santa Cruz Biotechnology | Cat# sc-365062; RRID:AB_10847862 |
Anti-MLKL (phospho S345) [EPR9515(2) mAb] | Abcam | Cat# ab196436; RRID:AB_2687465 |
Anti-double-stranded RNA (Clone J2) | SCICONS | Cat# 10010200; RRID:AB_2651015 |
Anti-PKR (phospho T446) antibody (Clone E120) | Abcam | Cat# ab32036; RRID:AB_777310 |
Anti-Z-DNA (Clone Z22 mAb) | Absolute antibody | Cat# Ab00783–3.0; RRID:AB_2820286 |
Anti-Vaccinia Virus polyclonal antibody | Abcam | Cat# ab35219; RRID:AB_778768 |
| ||
Chemicals, Peptides, and Recombinant Proteins | ||
| ||
Halt™ Protease and Phosphatase Inhibitor Cocktail | Thermo Scientific | Cat# 78446 |
SUPERase•In™ RNase Inhibitor | Invitrogen | Cat# AM2694 |
TRIzol™ Reagent | Invitrogen | Cat# 15596026 |
zVAD-fmk | SM Biochemicals | Cat# SMFMK001 |
GSK’872 | Sigma-Calbiochem | Cat# 5303890001 |
Istatin-b-thiosemicarbazone (IBT) | Pfaltz & Baur | Cat# I04639 lot 14888 |
Cycloheximide | Sigma-Aldrich | Cat# 01810 |
Hydroxyurea | Sigma-Aldrich | Cat# H8627 |
Actinomycin D | Sigma-Aldrich | Cat# A9415 |
Cytosine β-D-arabinofuranoside (AraC), | Sigma-Aldrich | Cat# C1768 |
Alpha-interferon, mouse, recombinant, E. coli. | Millipore Sigma | Cat# 407293 |
Recombinant Murine TNF-α | Peprotech | Cat# 315–01A |
Fixation/Permeabilization Solution Kit | BD Bioscience | Cat# 554714 |
ON-TARGETplus Mouse Zbp1 siRNA | Horizon | Cat# L-048021–00-0020 |
ON-TARGETplus Non-targeting Control Pool | Horizon | Cat# D-001810–10-05 |
Proteinase K | New England BioLabs | Cat# P8107 |
RNase A | Thermofisher | Cat# EN0531 |
RNase T1 | Thermofisher | Cat# EN0541 |
Fixable Viability Stain 700 | BD Horizon | Cat# 564997 |
DNase I | Thermofisher | Cat# 90083 |
SYTOX™ Green Nucleic Acid Stain | Invitrogen | Cat# S7020 |
DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | Invitrogen | Cat# D1306 |
INTERFERin transfection reagent | Polyplus | Cat# 409–10 |
MAXblock Blocking Medium | Active Motif | Cat# 15252 |
ProLong Gold Antifade Reagent | Thermo Fisher | Cat# P36930 |
Mouse Interferon Beta | PBL Assay Science | Cat# 12401–1 |
CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat# G7570 |
BS3 (bis(sulfosuccinimidyl)suberate) | Thermo Fisher | Cat# 21580 |
Dynabeads™ M-280 Sheep Anti-Mouse IgG | Thermo Fisher | Cat# 11202D |
| ||
Cells and Virus | ||
| ||
L929 (NCTC clone 929) | ATCC | ATCC® CCL-1 |
HeLa cells | Gift from George Pavlakis, NCI | Bert Jacobs |
SVEC4–10 derived EV and FLAG-ZBP1 | https://doi.org/10.15252/embr.201743947 | Jason Upton |
Vaccinia virus (VACV) |
https://doi.org/10.1073/pnas.0431131100
https://doi.org/10.1128/JVI.69.10.6605-6608 https://doi.org/10.1128/JVI.75.2.850-856 https://doi.org/10.1006/viro.1996 https://doi.org/10.1016/j.virol.2005.01.006 |
Bert Jacobs |
Monkey poxvirus | BEI Resources | (MPXV) 7–61 (WRAIR) |
| ||
Software and Algorithms | ||
| ||
Prism9 | https://www.graphpad.com/ | Version 9.1.0 (216) |
FlowJo | Becton Dickinson & Company (BD) | Version FlowJo 10.7.1 |
Clustal Omega | EMBL-EBI | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
Leica LAS X software | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| ||
Other | ||
| ||
BD FACSymphony™ A3 Flow cytometer | BD | A3 |
Leica SP8 instrument confocal microscope | Leica Microsystems | SP8 |