KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Anti-ZBP1, mAb (Zippy-1) | Adipogen | Cat# AG-20B-0010-C100; RRID:AB_2892147 |
| DYKDDDDK Tag (D6W5B) Rabbit mAb | Cell Signaling | Cat# 14793; RRID:AB_2572291 |
| Monoclonal Anti-Vaccinia Virus E3L, Clone TW2.3 (produced in vitro), NR-4547 | BEI Resources, NIAID, NIH | Cat# NR-4547; RRID:AB_2892148 |
| E3L Rabbit polyclonal Ab | Generated in Jacobs Lab | 10.1016/0042-6822(91)90768-7; RRID:AB_2892150 |
| Anti-β-Actin (Clone AC-74 mAb) | Sigma | Cat# A5316; RRID:AB_476743 |
| Anti-GAPDH (Clone G-9 mAb) | Santa Cruz Biotechnology | Cat# sc-365062; RRID:AB_10847862 |
| Anti-MLKL (phospho S345) [EPR9515(2) mAb] | Abcam | Cat# ab196436; RRID:AB_2687465 |
| Anti-double-stranded RNA (Clone J2) | SCICONS | Cat# 10010200; RRID:AB_2651015 |
| Anti-PKR (phospho T446) antibody (Clone E120) | Abcam | Cat# ab32036; RRID:AB_777310 |
| Anti-Z-DNA (Clone Z22 mAb) | Absolute antibody | Cat# Ab00783–3.0; RRID:AB_2820286 |
| Anti-Vaccinia Virus polyclonal antibody | Abcam | Cat# ab35219; RRID:AB_778768 |
|
| ||
| Chemicals, Peptides, and Recombinant Proteins | ||
|
| ||
| Halt™ Protease and Phosphatase Inhibitor Cocktail | Thermo Scientific | Cat# 78446 |
| SUPERase•In™ RNase Inhibitor | Invitrogen | Cat# AM2694 |
| TRIzol™ Reagent | Invitrogen | Cat# 15596026 |
| zVAD-fmk | SM Biochemicals | Cat# SMFMK001 |
| GSK’872 | Sigma-Calbiochem | Cat# 5303890001 |
| Istatin-b-thiosemicarbazone (IBT) | Pfaltz & Baur | Cat# I04639 lot 14888 |
| Cycloheximide | Sigma-Aldrich | Cat# 01810 |
| Hydroxyurea | Sigma-Aldrich | Cat# H8627 |
| Actinomycin D | Sigma-Aldrich | Cat# A9415 |
| Cytosine β-D-arabinofuranoside (AraC), | Sigma-Aldrich | Cat# C1768 |
| Alpha-interferon, mouse, recombinant, E. coli. | Millipore Sigma | Cat# 407293 |
| Recombinant Murine TNF-α | Peprotech | Cat# 315–01A |
| Fixation/Permeabilization Solution Kit | BD Bioscience | Cat# 554714 |
| ON-TARGETplus Mouse Zbp1 siRNA | Horizon | Cat# L-048021–00-0020 |
| ON-TARGETplus Non-targeting Control Pool | Horizon | Cat# D-001810–10-05 |
| Proteinase K | New England BioLabs | Cat# P8107 |
| RNase A | Thermofisher | Cat# EN0531 |
| RNase T1 | Thermofisher | Cat# EN0541 |
| Fixable Viability Stain 700 | BD Horizon | Cat# 564997 |
| DNase I | Thermofisher | Cat# 90083 |
| SYTOX™ Green Nucleic Acid Stain | Invitrogen | Cat# S7020 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | Invitrogen | Cat# D1306 |
| INTERFERin transfection reagent | Polyplus | Cat# 409–10 |
| MAXblock Blocking Medium | Active Motif | Cat# 15252 |
| ProLong Gold Antifade Reagent | Thermo Fisher | Cat# P36930 |
| Mouse Interferon Beta | PBL Assay Science | Cat# 12401–1 |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat# G7570 |
| BS3 (bis(sulfosuccinimidyl)suberate) | Thermo Fisher | Cat# 21580 |
| Dynabeads™ M-280 Sheep Anti-Mouse IgG | Thermo Fisher | Cat# 11202D |
|
| ||
| Cells and Virus | ||
|
| ||
| L929 (NCTC clone 929) | ATCC | ATCC® CCL-1 |
| HeLa cells | Gift from George Pavlakis, NCI | Bert Jacobs |
| SVEC4–10 derived EV and FLAG-ZBP1 | https://doi.org/10.15252/embr.201743947 | Jason Upton |
| Vaccinia virus (VACV) |
https://doi.org/10.1073/pnas.0431131100
https://doi.org/10.1128/JVI.69.10.6605-6608 https://doi.org/10.1128/JVI.75.2.850-856 https://doi.org/10.1006/viro.1996 https://doi.org/10.1016/j.virol.2005.01.006 |
Bert Jacobs |
| Monkey poxvirus | BEI Resources | (MPXV) 7–61 (WRAIR) |
|
| ||
| Software and Algorithms | ||
|
| ||
| Prism9 | https://www.graphpad.com/ | Version 9.1.0 (216) |
| FlowJo | Becton Dickinson & Company (BD) | Version FlowJo 10.7.1 |
| Clustal Omega | EMBL-EBI | https://www.ebi.ac.uk/Tools/msa/clustalo/ |
| Leica LAS X software | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
|
| ||
| Other | ||
|
| ||
| BD FACSymphony™ A3 Flow cytometer | BD | A3 |
| Leica SP8 instrument confocal microscope | Leica Microsystems | SP8 |