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. 2022 Jul 8;3(4):zqac033. doi: 10.1093/function/zqac033

Figure 5.

Figure 5.

Effect of cannabinoids on NFAT activity in standard culture medium. Pure cannabinoids were tested at 5 µm against NFAT activity. Jurkat T cells expressing NFAT reporter gene were pretreated with the cannabinoids before stimulation using 1 µm ionomycin and 20 ng/mL PMA. Bioluminescence emitted by NFAT-mediated luciferase expression was measured in arbitrary units 5 h poststimulation. All data points represent mean ± SEM of 3 independent runs (N = 3), with each run including n = 3 replicates per condition. *P< .05, **P< .01. Key: NT = nontreated cells, NS = nonstimulated, MeOH = methanol, MeCN = acetonitrile, DMSO = dimethylsulfoxide.