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. 2022 Jul 28;12:12955. doi: 10.1038/s41598-022-12683-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ECM deposited by cytokine-treated hRMEC causes induction of adhesion molecules in naïve hRMEC. Naïve hRMEC were plated onto ECM derived from TNFα- or IL-1β-treated hRMEC. Cells were collected and assayed for expression of (A) E-selectin (gene name: SELE), (B) ICAM-1, or (C) VCAM-1. Expression data are reported as fold induction over vehicle with bars representing mean ± SD (vehicle: n = 6; TNFα: n = 9; IL-1β: n = 9).