Table 2.
Validation results.
| 5-PT | DHEA | Cortisone | Cortisol | α-corto-lone | THS | Etio | Preg-nenolone | PT | PD | 5-PD | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Absolute matrix effekt (%) | 97.3 | 97.9 | 99.0 | 98.3 | 97.2 | 102.0 | 96.4 | 101.1 | 98.5 | 100.1 | 96.4 |
| Relative matrix effekt (%) | 99.5 | 97.9 | 100.8 | 100.2 | 98.8 | 99.7 | 101.5 | 96.4 | 98.9 | 101.6 | 98.3 |
| Absolute recovery (%) | 116.5 | 117.1 | 121.4 | 120.1 | 120.1 | 120.7 | 116.1 | 108.9 | 111.8 | 106.0 | 113.6 |
| Relative recovery (%) | 99.2 | 99.8 | 101.3 | 101.7 | 115.0 | 98.3 | 101.7 | 101.6 | 98.2 | 101.2 | 107.4 |
| Inter-day accuracy (%) | 97.8 | 100.6 | 100.3 | 99.6 | 101.7 | 99.8 | 101.9 | 99.3 | 101.2 | 100.2 | 98.9 |
| Inter-day accuracy LLOQ (%) | 105.1 | 100.7 | 102.0 | 102.3 | 98.7 | 99.6 | 102.4 | 112.4 | 100.3 | 100.5 | 109.4 |
| Intra-day accuracy (%) | 100.3 | 99.1 | 100.0 | 99.3 | 95.1 | 97.2 | 99.4 | 102.3 | 98.9 | 100.6 | 102.8 |
| Intra-day accuracy LLOQ (%) | 109.0 | 107.1 | 100.2 | 94.6 | 91.4 | 101.1 | 107.3 | 106.7 | 98.7 | 105.2 | 111.4 |
| Inter-day precision (%CV) | 2.3 | 2.6 | 2.0 | 1.9 | 2.4 | 8.3 | 2.9 | 3.4 | 2.5 | 2.4 | 2.7 |
| Inter-day precision LLOQ (%CV) | 3.3 | 2.7 | 2.1 | 4.3 | 2.8 | 10.1 | 3.3 | 2.4 | 3.0 | 2.4 | 2.8 |
| Intra-day precision (%CV) | 3.3 | 2.8 | 2.2 | 2.1 | 3.0 | 8.8 | 3.3 | 3.6 | 2.9 | 3.2 | 3.3 |
| Intra-day precision LLOQ (%CV) | 3.8 | 2.5 | 2.1 | 1.6 | 2.0 | 7.1 | 1.4 | 2.1 | 5.6 | 2.8 | 3.9 |
| LOD (ng/mL) | 2.0 | 2.0 | 1.0 | 1.0 | 5.0 | 4.0 | 5.0 | 2.5 | 2.0 | 5.0 | 10.0 |
| Dilution Integrity (%) | 103.7 | 104.8 | 108.7 | 109.4 | 104.6 | 105.2 | 108.0 | 107.1 | 107.9 | 111.3 | 115.2 |
| Reinjection reproducibility (%CV) | 0.9 | 1.2 | 0.9 | 1.0 | 1.6 | 5.8 | 2.0 | 3.4 | 2.2 | 1.6 | 2.7 |
| Linearity, R2, n = 5 (mean, SD) | 0.9963 (0.0022) | 0.9993 (0.0005) | 0.9998 (0.0002) | 0.9995 (0.0003) | 0.9992 (0.0009) | 0.9947 (0.0067) | 0.9994 (0.0004) | 0.9963 (0.0023) | 0.9996 (0.0003) | 0.9994 (0.0002) | 0.9940 (0.0015) |