Table 6.
Antioxidant parameters in serum of hens.
Groups |
P-value |
|||||||
---|---|---|---|---|---|---|---|---|
Item | C | N1 | N2 | N3 | SEM | ANOVA | Linear | Quadratic |
58 W | ||||||||
GSH (μmol/L) | 2.29 | 2.37 | 2.42 | 2.44 | 0.228 | 0.913 | 0.495 | 0.822 |
CAT (U/mL) | 4.31a | 4.64b | 5.02c | 5.04c | 0.165 | <0.001 | <0.001 | 0.189 |
T-SOD (U/mL) | 86.02 | 86.49 | 86.60 | 86.74 | 1.128 | 0.927 | 0.529 | 0.835 |
T-AOC (U/mL) | 8.74a | 9.28ab | 9.96b | 9.97b | 0.422 | 0.013 | 0.002 | 0.374 |
MDA (nmol/mL) | 12.94 | 12.46 | 12.14 | 12.04 | 0.622 | 0.457 | 0.131 | 0.673 |
H2O2 (mmol/L) | 123.65 | 118.86 | 115.38 | 114.91 | 4.472 | 0.196 | 0.042 | 0.497 |
62 W | ||||||||
GSH (μmol/L) | 2.05a | 2.13a | 2.77b | 2.74b | 0.172 | <0.001 | <0.001 | 0.610 |
CAT (U/mL) | 4.10a | 4.39ab | 4.75b | 4.73b | 0.175 | 0.001 | <0.001 | 0.209 |
T-SOD (U/mL) | 80.41a | 85.01b | 85.11b | 85.77b | 1.637 | 0.007 | 0.003 | 0.096 |
T-AOC (U/mL) | 7.59a | 8.88b | 9.25b | 9.31b | 0.445 | 0.001 | <0.001 | 0.057 |
MDA (nmol/mL) | 16.17a | 14.63b | 13.16c | 13.12c | 0.654 | <0.001 | <0.001 | 0.112 |
H2O2 (mmol/L) | 126.53a | 118.25ab | 110.06b | 110.12b | 4.608 | 0.002 | <0.001 | 0.207 |
Mean values within a row with different superscripts letters denote statistical significantly difference (P < 0.05, n = 12). Abbreviations: C, basal diet group; CAT, catalase; GSH, glutathione; H2O2, Hydrogen peroxide; MDA, malondialdehyde; N1, N2, and N3, basal diets supplemented with 0.1%, 0.2%, and 0.4% of naringin, respectively; T-AOC, total antioxidant capacity; T-SOD, total superoxide dismutase.