Table 1.

RMS error of microbead localization for varying SNR and number of microbeads (5 μm diameter; volume is set to yield high concentration of 2270 beads/mm³ and strong shadow density⁴⁴ equal to 5675 for 10 beads case). RMS XY was calculated as: $\text{RMS}\left(\sqrt{{\left(\text{x}-{\text{x}}_{\text{ideal}}\right)}^2+{\left(\text{y}-{\text{y}}_{\text{ideal}}\right)}^2}\right)$.

SNR	10 beads		25 beads		50 beads		100 beads
	RMS XY (μm)	RMS Z (μm)	RMS XY (μm)	RMS Z (μm)	RMS XY (μm)	RMS Z (μm)	RMS XY (μm)	RMS Z (μm)
Inf	1.5	1.58	1.64	2.47	1.66	3.16	1.93	3.45
20	1.53	1.69	1.65	2.80	1.67	3.34	2.03	3.63
10	1.55	2.25	1.69	3.03	1.71	3.65	2.10	3.80
5	1.59	2.71	1.63	3.63	1.69	3.6	2.10	3.69