FIGURE 2.

Lack of effects of resistin on the neurally-evoked contractile responses in the presence of L-NNA. (A): representative trace showing the EFS-evoked contractile responses at 4 and 8 Hz stimulation frequency (left hand panel). Addition of 2 × 10⁻⁴ M L-NNA to the bath medium enhances the amplitude of the neurally-evoked contractile responses (middle panel). In the presence of L-NNA, resistin (60 ng/ml) no longer depresses the amplitude of the EFS-evoked contractile responses (right hand panel). (B): bar chart showing the lack of effects of resistin on the mean amplitude of the neurally-evoked contractile responses in the presence of L-NNA. Amplitude of contractile responses is expressed as percentage of the muscular contraction induced by 2 × 10⁻⁶ M methacholine, taken as 100%. All values are means ± SEM of 8 strips (from 4 animals). *p < 0.05 L-NNA vs its own Ctrl; **p < 0.05 L-NNA + resistin vs its own Ctrl and p > 0.05 L-NNA + resistin vs L-NNA (ANOVA with Bonferroni’s post hoc test).