Table 2.
Sequencing analysis of potential off-target loci in gene-corrected dogs.
| Off-target | Sequences | Ch | Position | Dir | Mismatches | |
|---|---|---|---|---|---|---|
| gRNA | GAGGGGAACACACGCAGGACNGG | 4 | 38226314 | + | 0 | |
| #1 | Reference | GAGGGGAACACACGGAGGAAGGG | 15 | 5224102 | + | 2 |
| C>T Dog #1 | GAGGGGAACACACGGAGGAAGGG | |||||
| C>T Dog #2 | GAGGGGAACACACGGAGGAAGGG | |||||
| #2 | Reference | GAGGGGAAGTCAGGCAGGACAGG | 1 | 3887327 | + | 3 |
| C>T Dog #1 | GAGGGGAAGTCAGGCAGGACAGG | |||||
| C>T Dog #2 | GAGGGGAAGTCAGGCAGGACAGG | |||||
| #3 | Reference | GGGGGGGACACCCGCAGGACAGG | 4 | 33314482 | − | 3 |
| C>T Dog #1 | GGGGGGGACACCCGCAGGACAGG | |||||
| C>T Dog #2 | GGGGGGGACACCCGCAGGACAGG | |||||
| #4 | Reference | GAGGGGACCACAAGCAGGAGGGG | 22 | 38923250 | − | 3 |
| C>T Dog #1 | GAGGGGACCACAAGCAGGAGGGG | |||||
| C>T Dog #2 | GAGGGGACCACAAGCAGGAGGGG | |||||
| #5 | Reference | GAGGTGAAAACACGCTGGACAGG | 31 | 38989958 | + | 3 |
| Donor patient | GAGGTGAAAACATGCTGGACAGG | |||||
| C>T Dog #1 | GAGGTGAAAACATGCTGGACAGG | |||||
| C>T Dog #2 | GAGGTGAAAACATGCTGGACAGG |
Letters in bold indicate mismatches with the gRNA sequence. Ch: chromosome number; Dir: direction; Mismatch: number of mismatches.