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. 2022 Jul 28;5:753. doi: 10.1038/s42003-022-03712-2

Fig. 2. ASC treatment increased M2 macrophages and reduced neutrophils and M1 macrophages on day 3 and increased Tregs on day 7 in the kidney.

Fig. 2

a, b T-distributed stochastic neighbor embedding (tSNE) implementation for flow cytometry data visualization of leukocyte subsets and T-cell subsets. The data are presented as color-coded leukocyte population overlays (left) or cell number density color-coded (right) in the tSNE space. a Kidney samples on day 3 were divided into the following cell clusters: CD45+ cells, T cells, B cells, neutrophils, and M1 and M2 macrophages. b Kidney samples on day 7 were divided into the following clusters: CD45+ cells, non-CD3+ cells, CD8+ T cells, CD4+ T cells, and Tregs. c Frequency of renal leukocyte subsets in CD45+ cells analyzed by flow cytometry on days 3 and 7 (day 3, n = 5 per group; day 7, n = 8 per group). d The proportion of Ki67-positive cells in the renal Treg subset on day 3 (n = 3 per group). All data are shown as means ± SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 as determined by ANOVA.