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. 2022 Jul 28;5:753. doi: 10.1038/s42003-022-03712-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Experimental scheme for analyzing the distribution of administered ASCs in the body. DiD-dye labeled ASCs were administered to nephritis-induced WKY/NCrj rats on day 2. Tissues were harvested on day 3. b The number of DiD-positive cells with large DiD-dye particles and small DiD-dye particles in the liver, lung, spleen, and kidney is shown (n = 3 per group). Scale bars, 100 μm. c, d T-distributed stochastic neighbor embedding (tSNE) implementation for flow cytometry data visualization of leukocyte and T-cell subsets. The data are presented as color-coded leukocyte population overlays (left) or cell number density color-coded (right) in the tSNE space. e Frequency of splenic leukocyte subsets in CD45⁺ cells analyzed by flow cytometry on days 3 and 7 (day 3, n = 5 per group; day 7, n = 8 per group). f The population of Ki67-positive cells in splenic Treg subset on day 3 (n = 3 per group). All data are shown as means ± SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 as determined by ANOVA.