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. 2022 Jul 28;5:753. doi: 10.1038/s42003-022-03712-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Experimental scheme for analyzing the therapeutic potential of ASCs in rats treated with splenectomy. The rats underwent splenectomy 1 week before the nephrotoxic antibody injection. ASCs were administered on days 0, 2, and 4. b, c Serum creatinine level and histological crescent score on days 3 and 7 with and without splenectomy in each MSC treatment. d The tSNE plot shows the renal T-cell subset on day 3. e Frequency of renal leukocyte subsets in CD45⁺ cells analyzed by flow cytometry on days 3 and 7 (day 3, n = 4 per group; day 7, n = 7–8 per group). All data are shown as means ± SD. *p ≤ 0.05, ***p ≤ 0.001 as determined by Welch’s t test.