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. 2022 Jul 28;5:753. doi: 10.1038/s42003-022-03712-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Experimental scheme for analyzing the organ and cellular distribution of MSC membrane components by flow cytometry. The spleen and kidney were analyzed one day after the DiD-dye labeled ASC administration. The left graph shows the percentage of CD45-positive or negative subsets in DiD⁺ cells. The right graph shows the number of DiD⁺ cells in the spleen and kidney (n = 7 per group). b The confocal micrograph of CD11b/c⁺DiD⁺ cells sorted by flow cytometry. DiD particles were localized on the leukocyte membrane. c Representative images of ASC, Apoptotic ASC, and EV. Scale bars, 10 μm. The right graph shows the frequency of three DiD⁺ populations in the spleen and kidney (n = 3 per group). d tSNE plot from the flow cytometry analysis shows DiD⁺ leukocyte subsets. e Pie charts show the proportion of each subset in DiD⁺ leukocytes in the spleen and kidney (n = 7 per group). f Representative images and results of flow cytometry analysis show DiD⁺ cell frequency in renal M2 macrophages after administration of DiD-dye labeled ASCs or BMMSCs into nephritis rats (n = 7). All data are shown as means ± SD. *p ≤ 0.05 as determined by Welch’s t test.