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. 2022 Jul 28;5:753. doi: 10.1038/s42003-022-03712-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a The co-culture of whole bone marrow cells and ASCs. ASCs enhanced Treg induction in CD4⁺ T cells (n = 5 per group). b The co-culture of CD4⁺ T cells sorted from bone marrow and ASCs (n = 3 per group), c the co-culture of CD4⁺ T cells sorted from bone marrow and EVs derived from cultured ASCs (n = 3 per group), and d the co-culture of CD4⁺ T cells sorted from bone marrow and M2 macrophages with EVs (n = 4 per group) induced Tregs in CD4⁺ T cells. All data are shown as means ± SD. *p ≤ 0.05, **p ≤ 0.01, and **p ≤ 0.001 as determined by Welch’s t test.