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. 2022 Jul 28;12:12957. doi: 10.1038/s41598-022-16786-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design: probiotic experimental groups: A: Lactobacillus acidophilus CRL 2074; B: Limosilactobacillus fermentum CRL 2085; C: Limosilactobacillus mucosae CRL 2069; D: CRL 2085 + CRL 2069; E: CRL 2074 + CRL 2085 + CRL 2069; Control group (n = 21) included in a separate pen. Sampling time in days: 0 (T0), 40 (T1), 104 (T2) and 163 (T3). Experimetal periods of probiotics administration E-40, E-104 and E163. Sampling scheme: animals (n = 7) received probiotics up to 40 days and then removed (to separate pen) experimental period E-40; animals (n = 7) received probiotics up to 104 days and then removed, E-104 and, animals (n = 7) administered with probiotics for 163 days E-163. This scheme was replicated for the A, B, C, D and E experimental groups.