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. 2022 Jul 28;13(7):659. doi: 10.1038/s41419-022-05109-9

Fig. 1. Chronic HFD consumption decreases the percentage of ciliated cells in hypothalamic POMC neurons.

Fig. 1

A Body weight of C57BL/6 transgenic mice expressing eGFP in POMC neurons (POMC-eGFP) on a chow or high-fat diet (HFD) for 16 weeks. B Body weight (BW) increase of POMC-eGFP mice fed chow or HFD after 16 weeks. C Glucose tolerance test (GTT) and D area under the curve (AUC) of GTT of POMC-eGFP mice fed chow or high-fat diet (HFD) for 16 weeks. E Representative confocal images showing POMC-eGFP neurons and AC3 (adenylate cyclase 3, cilia) immunoreactivity in the arcuate nucleus of the hypothalamus, with the respective binary masks (F, G) of mice fed chow or HFD for 16 weeks. Arrowheads indicate cilia of POMC-eGFP neurons. Scale bar: 20 µm. Nuclei were stained with Hoechst (blue). H Percentage of ciliated cells in POMC-eGFP neurons. I Cilia length distribution of POMC-eGFP neurons from mice fed chow or HFD for 16 weeks. Data are presented as mean ± SEM. Statistical differences were evaluated by using: A Two-way ANOVA followed by post hoc Sidak multiple comparison test; B, D, H unpaired two-tailed Student t-test; C two-way ANOVA, post hoc Holm-Sidak multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n = 4/group.