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. 2022 Jul 15;10:866210. doi: 10.3389/fcell.2022.866210

FIGURE 3.

FIGURE 3

Akt is not responsible for mitochondrial fission under HFD treatment. (A) Immunostaining of mitochondria and F-actin of cardiomyocytes from ND- or HFD-treated control and Akt knockdown flies. Mitochondria are visualized using an anti-ATP5A1 antibody. Scale bar: 10 μm. (B) The proportion of the elongated mitochondria of the control and Akt knockdown flies under ND or HFD treatment. Two-way ANOVA: Interaction between diet and genotype is not significant, p = 0.7747. Tukey’s multiple comparison test: **p < 0.01, ***p < 0.001. N = 6∼8 (6∼8 hearts per genotype). The control group re-uses the data from Figure 1F. (C) The complex I activity of the control and Akt knockdown flies under ND or HFD treatment. Two-way ANOVA: Interaction between diet and genotype is not significant, p = 0.88. Tukey’s multiple comparison test: **p < 0.01, ns: not significant. N = 3 (3 replicates, 20 flies per replicate). (D) The quantification of the fractional shortening of control and Akt knockdown flies. Two-way ANOVA: Interaction between diet and genotype is not significant, p = 0.2564. Tukey’s multiple comparison test: *p < 0.05, ns: not significant. N = 9∼10 (9∼10 flies per condition).