FIG. 5.

Comparison of the sensitivity in the detection of target HAV RNA and HAV lysate between the developed NASBA using BB primers and RT-PCR using P primers. Tenfold serial dilutions of purified target RNA ranging from 400 to 0.04 ng/ml (A) and 10-fold serially diluted HAV lysate starting at 10⁶ PFU/ml (B) were prepared and subjected to either the RT-PCR (lanes 1) or NASBA (lanes 2). The detection limit is expressed as the minimum concentration of target RNA giving a detectable signal by dot blot hybridization.