TABLE 2.

The effect of nontarget microorganisms and nucleic acids on the specificity of the NASBA detection system

Organism or nucleic acid	Concn	No. positive/No. totala
E. coli ATCC-11775	2 × 107 CFU/ml	0/2
	2 × 106 CFU/ml	0/2
	2 × 105 CFU/ml	0/2
HAV (106 PFU/ml) + E. coli	2 × 107 CFU/ml	2/2
	2 × 106 CFU/ml	2/2
	2 × 105 CFU/ml	2/2
L. monocytogenes ATCC-43256	1 × 108 CFU/ml	0/2
HAV (106 PFU/ml) + L. monocytogenes	1 × 108 CFU/ml	2/2
Rotavirus	1 × 106 PFU/ml	0/2
	1 × 105 PFU/ml	0/2
	1 × 104 PFU/ml	0/2
HAV (106 PFU/ml) + rotavirus	1 × 106 PFU/ml	0/2
	1 × 105 PFU/ml	2/2
	1 × 104 PFU/ml	2/2
Yeast tRNA	50 μg/ml	0/2
HAV (106 PFU/ml) + yeast tRNA	50 μg/ml	2/2
E. coli rRNA	50 μg/ml	0/2
HAV (106 PFU/ml) + E. coli rRNA	50 μg/ml	2/2

^aNo. of replicates giving positive results in the combined NASBA-dot blot procedure per total number tested.