Table 1.
18-marker panel for imaging mass cytometry, with antibody-metal combination, antibody clone and staining conditions for tonsil and lung tissue.
| Target | Clone | Isotope | Tonsil | Lung | Species | ||||
|---|---|---|---|---|---|---|---|---|---|
| Time | Temp | Dilution | Time | Temp | Dilution | ||||
| pan-cytokeratin | AE1/AE3 and C11 | 89Y | ON | 4°C | 50 | ON | 4°C | 100 | RM, CM |
| CD45 | D9M8I | 115In | ON | 4°C | 100 | 5h | RT | 100 | RM, CM |
| HLADR | TAL-1B5 | 141Pr | 5h | RT | 100 | 5h | RT | 100 | RM, CM |
| CD4 | EPR6855 | 142Nd | 5h | RT | 100 | 5h | RT | 50 | RM, CM |
| CD11b | D6X1N | 143Nd | 5h | RT | 100 | 5h | RT | 100 | RM, CM |
| CD20 | H1 | 144Nd | 5h | RT | 100 | 5h | RT | 100 | RM, CM |
| CD16 | EPR16784 | 145Nd | 5h | RT | 100 | 5h | RT | 100 | RM, CM |
| CD8α* | D8A8Y | 146Nd | ON | 4°C | 50 | ON | 4°C | 50 | RM, CM |
| CD123 | IL3RA/2947R | 147Sm | 5h | RT | 50 | 5h | RT | 100 | RM, CM |
| γδTCR | H41 | 148Nd | 5h | RT | 50 | ON | 4°C | 100 | RM, CM |
| CD3ϵ | D7A6E | 156Gd | 5h | RT | 100 | 5h | RT | 100 | RM, CM |
| CD14 | D7A2T | 163Dy | ON | 4°C | 100 | ON | 4°C | 50 | RM, CM |
| Calprotectin | MAC387 | 171Yb | 5h | RT | 100 | ON | 4°C | 100 | RM, CM |
| CD206 | EPR22489-7 | 172Yb | 5h | RT | 100 | 5h | RT | 50 | RM, CM |
| CD7 | EPR4242 | 173Yb | ON | 4°C | 100 | ON | 4°C | 50 | RM, CM |
| CD68 | D4B9C | 174Yb | ON | 4°C | 100 | ON | 4°C | 50 | RM, CM |
| CD163 | Edhu-1 | 175Lu | 5h | RT | 100 | ON | 4°C | 50 | RM, CM |
| Histone H3 | D1H2 | 209Bi | ON | 4°C | 50 | ON | 4°C | 50 | RM, CM |
*Purchased conjugated format from Fluidigm.