Table 1.
Key resources
| Antibodies used for mice treatment | |||
| Treatment | Brand | Ref | Dose for each injection |
| InVivoMAb αmouse PD-1 (CD279) | BioXCell | BE0146 | 250 µg |
| InVivoMAb rat IgG2a isotype control, αtrinitrophenol (ISO for ΑPD1) | BioXCell | BE0089 | 250 µg |
| InVivoMAb αmouse CTLA-4 (CD152) | BioXCell | BE0164 | 100 µg |
| InVivoMAb mouse IgG2b isotype control, unknown (ISO for ΑCTLA-4) | BioXCell | BE0086 | 100 µg |
| InVivoMAb αmouse Ly6G | BioXCell | BE0075-1 | 100 µg |
| InVivoMAb rat IgG2a isotype control, αtrinitrophenol (ISO for ΑLy6G) | BioXCell | BE0089 | 100 µg |
| InVivoMAb αmouse CD25 (IL-2Rα) | BioXCell | BE0012 | 200–300 µg |
| InVivoMAb rat IgG1 isotype control, αhorseradish peroxidase (ISO for αCD25) | BioXCell | BE0088 | 200–300 µg |
| Antibodies used for fluorescence-activated cell sorting (FACS) analysis | |||
| Antigen | Ref | Fluorophore | Supplier |
| CD3 | 557 596 | APC-Cy7 | BD |
| CD3 | 562 600 | BV421 | BD |
| CD4 | 557 956 | APC-A700 | BD |
| CD8a | BLE100738 | BV421 | Ozyme |
| CD11b | 553 312 | APC | BD |
| CD11c | 560 583 | AA700 | BD |
| CD19 | 562 701 | BV421 | BD |
| CD25 | 558 642 | PE | BD |
| CD45 | 563 891 | BV510 | BD |
| FoxP3 | 17-5773-82 | APC | eBioscience |
| ICOS | 25-9942-82 | PE-Cy7 | eBioscience |
| KI67 | 561 284 | PC5.5 | BD |
| Ly6C | 560 593 | PC7 | BD |
| Ly6G | 560 600 | APC-Cy7 | BD |
| PD1 | BLE109112 | APC | Ozyme |
| NKp46 | 560 756 | FITC | BD |
| NKp46 | 562 850 | BV421 | BD |
| SiglecF | 552 126 | PE | BD |
CTLA-4, cytotoxic T-lymphocytes-associated protein 4; PD-1, programmed death 1.