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. 2022 Jul 15;13:957444. doi: 10.3389/fmicb.2022.957444

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Copyright © 2022 Zhang and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of culture conditions for cellulase production by microbial consortium VI by the single factor approach. In Panel (A), the temperature was 30°C, the pH was 7, and the inoculum volume was 5% (v/v); in Panel (B), the culture time was 4 days, the pH was 7, and the inoculum volume was 5% (v/v); in Panel (C), the temperature was 35°C, the culture time was 4 days, and the inoculum volume was 5% (v/v); in Panel (D), the temperature was 35°C, the culture time was 4 days, and the pH was 6.5. (A) Effect of time on enzyme production by consortium. (B) Effect of temperature on enzyme production by consortium. (C) Effect of initial pH on enzyme production by consortium. (D) Effect of inoculation volume on enzyme production by consortium. Error bars represent standard deviation; they were calculated using data from 3 technical replicates. Different letters (e.g., “a,” “b,” “c,” and “d”) represented significant difference (p < 0.05).