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Response surface test factors and levels.
| Level | A culture time (d) | B temperature (°C) | C pH | D inoculum volume (%) |
| −1 | 3 | 30 | 6.0 | 3 |
| 0 | 4 | 35 | 6.5 | 5 |
| 1 | 5 | 40 | 7.0 | 7 |
A, culture time; B, temperature; C, pH; D, inoculum volume. They are four factors in response surface test, as the independent variables.
