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RNA interference knockdown of Parkin transcript and protein abundance in flight muscle of diapausing Colorado potato beetles. Verification of transcript and protein knockdown via qPCR and Western blot, respectively, in beetles injected with dsParkin compared to beetles injected with a negative control dsRNA construct, dsGFP. All values are represented as ± SD. Asterisk denotes significant differences between treatments according to a Welch’s t-test (P < 0.05; SI Appendix, Table S2).
