Fig. 2.

Experimental validation of Yeast8 gene targets. (A) Heme production (fold-change) of 15 gene KO strains from the YKO collection (BY4741 strain background). BY4741 strain served as a control to normalize data (shown in green). Two replicates were used in the analysis. Heme was extracted from eight OD₆₀₀ of cells. The gene targets where heme production was higher than the control are highlighted in red. The gene targets where heme production was lower than the control are highlighted in blue. (B) Heme production (fold-change) of strains carrying 61 model genes overexpressed under control of the TEF1 promoter using a centromeric plasmid in CEN.PK113-11C strain background. Heme was extracted from eight OD₆₀₀ of cells. CEN.PK113-11C carrying empty vector served as a control to normalize data (shown in green). Two replicates were used in the analysis. (C) Heme production of strains with gene modifications that improved heme production the most. Average value of two replicates was used. Heme was extracted from eight OD₆₀₀ of cells. (D) Schematic overview of metabolism with Yeast8 targets, which experimentally improved heme production.