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Activity of the late promoter during induction of ORF29 synthesis. Strains LB560 containing pMBP14(Plate-lacLM) and pMBP22(PnisA-orf29-gusA) (A) and LB562 containing pMBP14(Plate-lacLM) and pNZ8010(PnisA-gusA) (B) were grown exponentially in M17 medium with 0.5% glucose and erythromycin and chloramphenicol (each, 5 μg/ml). At 5 min, nisin was added to a final concentration of 1 ng/ml. The specific activities of β-galactosidase (circles) and β-glucuronidase (triangles) were measured.
