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. 2022 Jul 22;119(30):e2203743119. doi: 10.1073/pnas.2203743119

Fig. 5.

Fig. 5.

The C-terminal domain of OSR1 interacts with occludin to influence tight junction turnover. (A) Representative Western blots show endogenous coimmunoprecipitation of OSR1 with occludin in primary HUVECs showing enhanced interaction of OSR1 and occludin upon overnight TGF-β (10 ng/mL) and WNK463 (1µM) treatment; n = 5. (B) Representative Western blots show endogenous coimmunoprecipitation of OSR1 and SMURF2 with occludin in HUVECs treated with WNK463 (1 µM) overnight, which is diminished upon coincubation with the blocking peptide SAGRRFIVSPVPE. (C) Corresponding quantification of OSR1 immunoprecipitated over occludin with or without the presence of blocking peptide; n = 5. (D) Representative Western blot show immunoprecipitation (IP) of FLAG-occludin overexpressed in HEK293 cells interacts with endogenous OSR1, which is blocked with coincubation with the blocking peptide SAGRRFIVSPVPE; n = 5. (E) Representative Western blots show immunoprecipitation of FLAG-occludin overexpressed in HEK293 cells and its interaction with myc-C-terminal OSR1.