Fig. 1.

NusG depletion enhances HilD-dependent SPI-1 bistability. The strains used, MA14302 (hilD⁺) and MA14561 (hilDΔ309), carry a hilA-gfp^SF translational gene fusion (hilA::gfp^SFΔK28) and a chromosomal P^Tac promoter-mCherry gene fusion in the ARA-inducible-NusG depletion background. (A) Representative image of MA14302 cells grown at 37 °C to early stationary phase visualized by fluorescence microscopy under 100× magnification. (B and C) Representative flow cytometry analysis of cells from strains MA14302 (B) and MA14561 (C) grown as in A. The GFP fluorescence intensity distribution was examined in strains carrying gfp translational fusions. The full genotypes of MA14302 and MA14561 are shown in SI Appendix, Table S1. For the construction of hilA::gfp^SFΔK28 and hilDΔ309 by λ red recombineering, DNA primers (listed in SI Appendix, Table S2) were used as detailed in SI Appendix, Table S3.