FIGURE 1.

Various cell types in the lung express aconitase 1 in a different subcellular localization pattern (A–C) Paraffin-embedded lung sections from wild-type mice aged 7–9 weeks were immunohistochemically labeled for i) Aconitase 1 (ACO1: (A,B) or ii) E-cad and ACO1 (C) (D–F) Paraffin-embedded lung sections from patients with idiopathic pulmonary fibrosis (IPF) were subjected to immunohistochemical labeling for i) ACO1 (D), ii) E-cad and ACO1 (E) or iii) E-cad, ACO1 and vWF (F) (G) Specificity of the ACO1 antibody used in this article (cat# PA5-41753, Thermo Fisher Scientific) was evaluated by western blot analysis using whole cell lysates from non-treated H441 cells and H441 cells transfected with either ACO1-overexpression or control plasmids. Equal amounts of protein (7.5 μg) were loaded per lane (H) The expressions of ACO1 in different cell types were examined by western blotting. PBEC (Human Pulmonary Bronchial Epithelial Cells); HMVEC (Human Lung Microvascular Endothelial Cells); T7 (mouse immortalized type II alveolar epithelial cell line); C22 (mouse immortalized club cell line); Fibroblast (primary mouse lung fibroblasts). Equal amounts of protein (7.5 μg) were loaded per lane. Data from one representative experiment of two or more independent experiments are shown.