FIG. 2.

Expression of nahases (N), endochitinase (E), and chitobiosidase (C) by T. atroviride as assessed by separation of enzymes using native PAGE and visualization of activity from culture filtrates. Filtrates were harvested from cultures grown in EMM containing different levels of ammonium acetate (H = 100 mM; L = 10 mM) and glucose (H = 3%; L = 0.1%) in the presence or absence of colloidal chitin (1%). Activity was assessed by release of methylumbelliferone by digestion of the appropriate methylumbelliferyl substrate. Culture filtrates (15 μl) were loaded for each lane and separated at constant voltage (4 V/cm).