FIG. 3.

Expression of 1,3-β-glucosidase by T. atroviride as assessed by separation of enzymes using native PAGE and visualization of activity from culture filtrates. Filtrates were harvested from cultures grown in EMM containing different levels of ammonium acetate (H = 100 mM; L = 10 mM) and glucose (H = 3%; L = 0.1%) in the presence or absence of colloidal chitin (1%). Activity was detected by a decrease in fluorescence of laminarin conjugates with aniline blue decolorized at pH 9.0. Culture filtrates (15 μl) were loaded for each lane and separated at constant voltage (4 V/cm). Shown are the 1,3-β glucosidase GLUC78 and another glucosidase that has been heretofore unknown.