Figure 1.

(A) Schematic of LNP synthesis including the components used to make LNPs via microfluidic mixing and the expected resulting structure. (B) Visualization of the design process used to generate libraries A and B with library A resulting from an orthogonal DOE screening a wide range of excipient molar ratios, and library B examining more formulations within a narrowed range of excipient ratios based on the results from the library A screen. (C) Schematic of the CAR T cell production utilizing either LNPs or EP for mRNA delivery to T cells. The treated T cell populations may differ in viability and CAR potency depending on the transfection method, but both are able to generate functional CAR T cells to induce targeted cancer cell killing.