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. 2001 Dec;67(12):5675–5682. doi: 10.1128/AEM.67.12.5675-5682.2001

FIG. 1.

FIG. 1

Different sizes of PCR products obtained with a primer pair targeting the conserved segments of class 1 integrons in motile aeromonads. Class 1 integrons were—if present—invariably cotransferred to E. coli on OTC resistance plasmids. Lanes a and m, 100-bp DNA marker; lane b, positive control S. enterica serovar Typhimurium DT104 with 1,000- and 1,200-bp amplicons; lane c, negative control; lane d, “empty” integron with no gene inserts between conserved ends; lanes e and f, isolate 1-75 and the corresponding E. coli transconjugant with 1,400-bp amplicons; lanes g and h, isolate 4-229 and transconjugant with 1,550-bp amplicons; lanes i and j, isolate 2-280 and transconjugant with 2,100-bp PCR products; lanes k and l, isolate 4-221 and transconjugant with 2,900-bp products. kb, kilobases.