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Overview of the SLOMO workflow for measuring the affinities (Kd) of biomolecular interactions. Step 1. Tip loading (with solution 1 and solution 2). Step 2. Time-resolved ESI-MS analysis. Step 3. Extraction of time-resolved abundances. Step 4. Calculation of the time-dependent relative RF. Step 5. Calculation of Kd from RF and Rapp. The insert summarizes biomolecular interactions used in this work and the range of Kd of interactions that are measurable with SLOMO.
