Table 1. Comparison of Affinities (K_d) of Three Biomolecular Interactions (Van–AcAA, PT–STI, and BLG–BLG) Measured by SLOMO nanoESI-MS with Literature Values^a.

system	apparent Kd (μM)	corrected Kd (μM)	reported Kd (μM)
Van–AcAAb	378 ± 36	32 ± 1	63 ± 13e
PT–STIc,d	0.2 ± 0.1	0.5 ± 0.1	0.67f
	28 ± 9	0.8 ± 0.3
BLG–BLGb	1.9 ± 0.8	3.0 ± 0.5	7.0g

^aIncluded are the apparent K_d (without correction for RF) and the corrected K_d (correction for RF) measured by SLOMO. Errors correspond to one standard deviation.

^bMeasurements were performed in ammonium acetate (200 mM, pH 6.9, and 25 °C) solutions.

^cMeasurements were performed in ammonium acetate (200 mM, pH 4.5, and 25 °C) solutions.

^dTwo sets of data were obtained for the same solutions using different instrumental parameters.

^eValue taken from ref (48). Measurements were performed in 100 mM phosphate buffer (pH 7.0, 25 °C).

^fValue taken from ref (9). Measurements were performed in 25 mM potassium acetate and 10 mM calcium chloride buffer (pH 4.5, 25 °C).

^gValue taken from ref (55). Measurements were performed in phosphate buffer (pH 6.9, ionic strength of 0.13).