Fig. 2.

Kockdown of PTPRO reduces capillary leakage and attenuates HS-induced lung injury. a Lung histopathology in the HS model was assessed by H&E staining. Magnification: 200 × . Scale = 100 μm. b Histologic score corresponding to H&E staining for lung injury assessment. c Micrograms of Evans blue (EB) per gram of lung tissue and per microlitre of bronchoalveolar lavage fluid (BALF) were used to assess lung permeability in HS model. d Relative mRNA levels of tight junction proteins zonula occludens-1 (ZO-1) and occludin (OCC) in the lung tissues were determined by RT-qPCR. e, f Representative blot image e. and relative protein expression f of ZO-1 and OCC in the lung tissues were detected by Western blot. Values are means (n = 6) ± SD. **P < 0.01