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. 2022 Jul 29;22:376. doi: 10.1186/s12870-022-03775-7

Table 2.

Primer sequences of genes encoding enzymes involved in 2-AP biosynthesis

Gene name Accession No Primer sequences
Proline dehydrogenase (PRODH) AP014966.1

F 5'-TCATCAGACGAGCAGAGGAGAACAGG-3'

R 5'-CCCAGCATTGCAGCCTTGAACC-3'

Pyrroline-5-carboxylic acid synthetase2 (P5CS2) AP014957.1

F 5'-GAGGTTGGCATAAGCACAG-3'

R 5'-CTCCCTTGTCGCCGTTC-3'

Ornithine aminotransferase (OAT) AP014959.1

F 5'-GCCCTTGGTGCTGGAGTA-3'

R 5'-AGCCCTTTCAACGAGACCTT-3'

Diamine oxidase2 (DAO2) AP014960.1

F 5'-TCGTTCGCATCAAGGTTGG-3'

R 5'-TCAGACAGAAGGGTGCCGTA-3'

Diamine oxidase4 (DAO4) AP014960.1

F 5'-TGGCAAGATAGAAGCAGAAGT-3'

R 5'-GTCCATACGGGCAACAAA-3'

Betaine aldehyde dehydrogenase (BADH2) AB09683

F 5'-GGTTGGTCTTCCTTCAGGTGTGC-3'

R 5'-CATCAACATCATCAAACACCACTAT-3'

Pyrroline-5-carboxylate reductase (P5CR) AP014957.1

F 5'-TGGGCTAAGTGGTAGTGGC-3'

R 5'-AGCTGACCCGGATGTTTT-3'