TABLE 3.
Inhibition of multiplex PCR by different concentrations of food and fecal material
| Concn of sample materiala (μg/PCR mixture) |
C. botulinum type(s) detected
|
||
|---|---|---|---|
| Minced beef | Fish | Feces | |
| 500 | A, B, E, F | A, B, E, F | |
| 250 | A, B, E, F | B, E, F | |
| 100 | B | A, B, E, F | A, B, E, F |
| 50 | B, E, F | A, B, E, F | A, B, E, F |
| 25 | B, E | A, B, E, F | A, B, E, F |
| 10 | A, B, E, F | A, B, E, F | A, B, E, F |
| 5 | A, B, E, F | A, B, E, F | A, B, E, F |
a
The actual level of sample material in the PCR was estimated to be 1 μg with a dilution (1:9) step in peptone water and was 10 μg without the dilution.