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Detection limit and optimal enrichment time of multiplex PCR assay for C. botulinum types A, B, E, and F in food and fecal samples
| C. botulinum type | Detection limit in three sample materials ([spores/g of sample material], optimal enrichment time [days])
|
||
|---|---|---|---|
| Minced beef | Hot-smoked whitefish | Feces | |
| A | 10−2, 5 | 10−1, 3 | 10, 3 |
| B | 10−2, 1 | 10−2, 3 | 10−1, 3 |
| E | 10−1, 3 | 10−1, 1 | 103, 5 |
| F | 10−1, 3 | 10−2, 5 | 10, 3 |
