FIGURE 6 |.
TNAP and ENPP1 ablation effects on long bone. Trabecular thickness (Tb.Th.), number (Tb.N.), spacing (Tb.S.), bone mineral density (BMD) and bone volume fraction (BVF) are shown (A–E). Cortical mean thickness, area, tissue mineral density (TMD), BMD and BVF are shown (F–J). TNAP−/−/ENPP1+/+ mice are diminished in trabecular thickness and mineral density with increased trabecular number and no difference in bone volume fraction as compared to TNAP+/+/ENPP1+/+ mice. TNAP−/−/ENPP1−/− mice are diminished in trabecular thickness, number and bone volume fraction and have increased trabecular spacing compared to TNAP+/+/ENPP1+/+ mice. No differences are seen between TNAP+/+/ENPP1−/− mice and TNAP+/+/ENPP1+/+ mice. *p < 0.05 vs. TNAP+/+/ENPP1+/+ mice, ***p < 0.005 vs. TNAP+/+/ENPP1+/+ mice, #p < 0.05 vs. TNAP−/−/ENPP1+/+ mice, ###p < 0.005 vs. TNAP−/−/ENPP1+/+ mice.