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. 2022 Jul 29;7:86. doi: 10.1038/s41541-022-00513-w

Fig. 7. IL-17A neutralization in LAMPs-vaccinated/Mp-challenged animals reduces inflammatory cytokines, neutrophil recruitment, and severity of histopathological lung lesions but impairs bacterial clearance.

Fig. 7

A Illustration of experimental timeline and outcome measures. BALF concentrations of B IL-17A, C TNF-α, D IL-1β, E IL-6, and F KC in LAMPs-vaccinated/Mp-challenged animals receiving an anti-IL-17A neutralizing monoclonal antibody (17F3) or isotype control (MOPC-21). BALF numbers of lung-infiltrating leukocytes (G), and proportion of H and number of (I) lung-infiltrating neutrophils. J Lung Lesion Scores and K bacterial loads of vaccinated-then-challenged animals treated with anti-IL-17A antibody or isotype control. *p < 0.5, **p < 0.1, ***p < 0.01, ****p < 0.001. Error bars for J and K indicate median and interquartile range and mean and SEM for B–I. Each point represents data from an individual animal. Nonparametric lesion score, bacterial burden and percent frequency/proportion data were analyzed via an unpaired, two-tailed Mann–Whitney U-test. Parametric cytokine concentration and cell count data were analyzed via an unpaired, two-tailed t-test.