Figure 2.

Heatmap depicting the predictive impact of substitution mutations for each residue of ORF10 amino-acid sequence shown at the top label of the figure. As seen from the scoring bar below the heatmap, dark red square (score >50) indicates a high score for the strong effect of a substitution mutation, white-colored square indicates weak signals (−50 <score <50), meaning that there may be an effect, and blue-colored square indicates a low score (score < 50), meaning that this substitution mutation is neutral or has no effect. Black-colored squares indicate the corresponding wild-type residues.