Table 1. Primer information for real-time PCR analysis.
| Gene | Sequence (5’ → 3’) | Tm (°C) | PCR product (bps) |
|---|---|---|---|
|
Cebpa (NM_001287523.1) |
(F)
AGAAGTCGGTGGACAAGAAC (R) CTTGACCAAGGAGCTCTCAG |
52 | 233 |
|
Cebpb (NM_009883.4) |
(F)
ATGCAATCCGGATCAAACGTG (R) CAACCCCGCAGGAACATCTTTA |
60 | 113 |
|
Cebpg (NM_009884.3) |
(F)
ACCCATGGATCGGAATAGTG (R) GCTTCCAACCGTTCATTCTC |
55 | 150 |
|
Srebp1 (NM_011480.3) |
(F)
AGGTCACCGTTTCTTTGTGG (R) TCCAAGGGCATCTGAGAACT |
55 | 208 |
|
Ar (NM_013476.4) |
(F)
TGTCAAAAGTGAAATGGGACC (R) TGGTACTGTCCAAACGCATGT |
55 | 74 |
|
Esr1 (AB560752.1) |
(F)
GCCTCTGGCTACCATTATGG (R) CATCTCTCTGACGCTTGTGC |
55 | 244 |
|
Esr2 (U81451.1) |
(F)
GTCAGGGTGGTTGGGTTGTGG (R) ATCGCGTCACTTTCCTTTTCC |
55 | 203 |
|
Rn18s (NM_03278.3) |
(F)
ACTCAACACGGGAAACCTCA (R) AACCAGACAAATCGCTCCAC |
55 | 123 |
PCR, polymerase chain reaction; Cebpa, CCAAT enhancer binding protein alpha; Cebpb, CCAAT enhancer binding protein beta; Cebpg, CCAAT enhancer binding protein gamma; Srebp1, sterol regulatory element binding transcription factor 1; Ar, an-drogen receptor; Esr1, estrogen receptor alpha; Esr2, estrogen receptor beta; Rn18s, 18s ribosomal RNA.