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. 2020 Apr 9;21(6):e49783. doi: 10.15252/embr.201949783

Figure EV2. (Related to Fig 1). Expression of the myelin‐associated proteins in PLP‐23Q mice treated with LAQ.

Figure EV2

  1. Western blotting showing the multiple myelin proteins (MBP, MOBP, and MOG) in the corpus callosum in PLP‐23Q mice that were treated with LAQ (5 or 25 mg/kg) at 3 months of age for 2 months. Vinculin served as a loading control.
  2. Quantitative PCR analysis of myelin‐associated gene (MBP and MOG) in the corpus callosum of LAQ (5 or 25 mg/kg)‐treated PLP‐23Q mice. n = 3 each group. Data are mean ± SEM.
  3. Western blotting with anti‐HTT (EM48) showing the aggregated and soluble huntingtin in the corpus callosum in PLP‐150Q mice that were treated with LAQ (5 mg/kg) at 3 months of age for 2 months. Beta‐actin served as a loading control.
  4. Co‐transfection of N‐terminal MYRF (nMYRF) with N‐terminal HTT containing 150Q or 23Q in HEK293 cells and immunoprecipitation of nMYRF. More HTT‐150Q binds nMYRF than HTT‐23Q. Five μM LAQ treatment could decrease the immunoprecipitated HTT. The ratio of precipitated HTT to input in transfected cells treated with DMSO or LAQ obtained from three independent experiments was shown on the right. One‐way ANOVA followed with Tukey's test. ***P = 1.25 × 10−5; **P = 0.00552. Data are mean ± SEM.