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. 2020 Apr 9;21(6):e49783. doi: 10.15252/embr.201949783

Figure EV4. (Related to Figs 4 and 5). Mutant HTT promotes PRKG2 gene transcript expression.

Figure EV4

  1. N2a cells transfected with 23Q‐HTT or 150Q‐HTT were examined by the antibody of PRKG2 or phosphor‐VASP (pVASP). Note that both the expression of PRKG2 and pVASP was increased by 150Q‐HTT. GAPDH served as control. Ratios of PRKG2 or phosphorylated VASP (p‐VASP) to GAPDH obtained from three independent experiments were presented on the right. One‐way ANOVA followed with Tukey's test. PRKG2: ***P = 0.00032; p‐VASP: ***P = 0.00059. Data are mean ± SEM.
  2. The co‐transfection of 150Q‐HTT with core promoter of PRKG2 (−472/+131) displayed an obvious induction of the transcription activity from three independent experiments, compared with the 23Q‐HTT or Mock control. One‐way ANOVA followed with Tukey's test. ***P < 0.001. Data are mean ± SEM.
  3. Western blotting of transfected N2a cells showing that AhR siRNA, but not the scrambled control siRNA, treatment for 72 h inhibited AhR protein expression. The blots were probed with anti‐AhR antibody.