Table 1.
Primer sequences for plasmid constructs, qPCR, and ChIP assay
| Name | Forward primer | Reversed primer |
|---|---|---|
| PLP‐polyCAG | 5′‐ATGAAGGCCTTCGAGTCCCTCAAGTCCTTC‐3′ | 5′‐AAACTCACGGTCGGTGCAGCGGCTCCTCAG‐3′ |
| Cas9‐WT | 5′‐AAGGGAGCTGCAGTGGAGTA‐3′ | 5′‐TCGAAAATCTGTGGGAAGTC‐3′ |
| Cas9‐Mutant | 5′‐AAGGGAGCTGCAGTGGAGTA‐3′ | 5′‐TGGGCCATTTACCGTAAGTTAT‐3′ |
| nMYRF‐Myc | 5′‐ACATCGATACCATGGAACAAAAGCTCATTAGCGAGGAAGACCTTATGGAGGTGGTGGACGAGAC‐3′ | 5′‐TCTTGTCGACCTAGGTGGTGTCCACCTCCTGCACGTGCTC‐3′ |
| nMYRF‐GST | 5′‐AGAATTCGCCATGGAGGTGGTGGACGAGACCGAAGCGCTGCAG‐3′ | 5′‐TCTTGTCGACCTAGGTGGTGTCCACCTCCTGCACGTGCTC‐3′. |
| nMYRF‐S259A | 5′‐AAGAGGAAGCACGCAGAATCACCCCCCAAC‐3′ | 5′‐CTTAGAGGGGTGTGGAGGGAGTTCAGCTC‐3′ |
| nMYRF‐S261A | 5′‐AAGCACTCTGAAGCACCCCCCAACACCCTC‐3′ | 5′‐CCTCTTCTTAGAGGGGTGTGGAGGGAGTT‐3′. |
| Promoter‐MBP | 5′‐ATGGTACCCTGTGTGAGCATGTGACA‐3′ | 5′‐TTTAAGCTTGAACAGTCCCCGTGAGG‐3′. |
| PRKG2‐1209 | 5′‐CCGCTCGAGTTTTACCTCAATGGACAGAGAGTAC‐3′ | 5′‐CCCAAGCTTAGCTCAGCGGAGAGGAGGGACGGAGG‐3′ |
| PRKG2‐866 | 5′‐CCGCTCGAGTCATTCTATGTGGGGTTATGTATGT‐3′ | 5′‐CCCAAGCTTAGCTCAGCGGAGAGGAGGGACGGAGG‐3′ |
| PRKG2‐472 | 5′‐CCGCTCGAGGAATCAGGAGGTATTAATTCCTGTC‐3′ | 5′‐CCCAAGCTTAGCTCAGCGGAGAGGAGGGACGGAGG‐3′ |
| PRKG2‐161 | 5′‐CCGCTCGAGATGATGAATACCCCCCTCAACTTTC‐3′ | 5′‐CCCAAGCTTAGCTCAGCGGAGAGGAGGGACGGAGG‐3′ |
| PRKG2‐cDNA | 5′‐CCGGAATTCATGGGAAATGGTTCAGTGAAGCCCAAGCATG‐3′ | 5′‐CCCAAGCTTTCAGAAGTCTTTATCCCAGCCTGACATTTC‐3′ |
| MBP‐qPCR | 5′‐AGTACCTGGCCACAGCAAGT‐3′ | 5′‐AGGATGCCCGTGTCTCTGT‐3′ |
| MOG‐qPCR | 5′‐ACGGGCATGGAGGTGGGTT‐3′ | 5′‐CAGGTGCTTGCTCTGCATCTTG‐3′ |
| GAPDH‐qPCR | 5′‐AACTTTGTCAAGCTCATTTCCTGGT‐3′ | 5′‐GGTTTCTTACTCCTTGGAGGCCATG‐3′ |
| Cyp1a1‐qPCR | 5′‐CGTGAGCAAGGAGGCTAACTAT‐3′ | 5′‐TGGCTACTGACACGACCAAATAC‐3′ |
| Ugt1a6a‐qPCR | 5′‐GTCCCTACCTTCCTTACACCAG‐3′ | 5′‐TCAGCTTTCCCTTCTTCTTACAG‐3′ |
| PRKG1‐qPCR | 5′‐ACCCTTGGAGTTGGAGGTTTCG‐3′ | 5′‐TGGCGTTTCTTGAGGATCTTCATT‐3′ |
| PRKG2‐qPCR | 5′‐TACGGGAGAAACTATCAACAGG‐3′ | 5′‐GGAACACCTCAAGTCTACCCTC‐3′ |
| AhR‐ChIP‐1 | 5′‐AGTCCTAGAAGAGCACGCGGGCCA‐3′ | 5′‐GTGGGCTCTTGCTCTCCCTTGCT‐3′ |
| AhR‐ChIP‐2 | 5′‐CCTCTCCCTCGGCCCACACACTTG‐3′ | 5′‐GGCGACGGCGACAGTCTGCACTTTA‐3′ |
| AhR‐ChIP‐34 | 5′‐CCCCACCTTCAAGGAAGCGGGCT‐3′ | 5′‐AGCTCAGCGGAGAGGAGGGACGGA‐3′ |
| Cas9‐PRKG2 | 5′‐ACTGCCGTGTGTCTGTTTATGAAGC‐3′ | 5′‐CAGCATCCAGTGGCAATTCCAGATC‐3′ |