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. 2022 Jul 27;14(1):2105637. doi: 10.1080/19490976.2022.2105637

Figure 4.

Figure 4.

Butyrate acts through its iHDAC activity to activate AhR in synergie with AhR ligands. (a) AhR activity in HepG2luc treated in triplicate with butyrate (1 mM), FICZ (10 ng/ml), TSA (100 nM) or the combination of FICZ with butyrate or TSA in a FBS-free media. Data are presented as mean ± SEM of three independent experiments. (b) AhR binding to the promoter region of the CYP1A1 gene detected by ChIP-qPCR in HepG2 treated with DMSO, FICZ and butyrate at several time points. (c) Synergistic recruitment of AhR to the CYP1A1 promoter mediated by butyrate in combination with FICZ. Only experiments with an enrichment greater than or equal to 1.5 for FICZ were retained. Data are presented as mean ± SEM and each dot represent an independent experiment. (d) RT-qPCR analysis of CYP1A1 mRNA levels in HepG2 treated with butyrate (1 mM), FICZ (10 ng/ml), C646 (10 µM) or the combination of FICZ with butyrate or C646 for 8 hours. Data are presented as mean ± SEM of two independent experiment. **p < .01; ***p < .001; ****p < .0001.