Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 15;13:892701. doi: 10.3389/fimmu.2022.892701

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Sampath, Natarajan, Moideen, Kathamuthu, Hissar, Dhanapal, Jayabal, Ramesh, Tripathy, Ranganathan, Babu and Bethunaickan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Representative gating strategy for dendritic cells and their subsets. Monocyte and granulocyte population were gated on whole blood cell plot excluding lymphocytes by Forward (FSC-A) and Side scatter (SSC-A) based on its size and granularity. Singlets were discriminated from doublets by FSC (A) vs FSC (H). Lineage negative cells were identified with the help of dump gate by excluding T cells, B cells and NK cells using CD3, CD19 and CD56 markers. HLA-DR+ were gated to obtain HLA-DR⁺CD11c⁺ myeloid DCs (mDC), cross-presenting HLA-DR⁺CD14^-CD141⁺ myeloid DCs and HLA-DR⁺CD14^-CD16^-CD11c^-CD123⁺plasmacytoid DCs (pDC).