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. 2022 Jun 6;4(3):fcac144. doi: 10.1093/braincomms/fcac144

Figure 2.

Figure 2

All surveyed mutant GAT-1 transporters had less mature but more immature form of the GAT-1 protein. (A, B) The total lysates of HEK293T cells expressing the wildtype or variant GAT-1 were undigested (U) or digested with Endo-H (H) and then analysed by SDS-PAGE. The membrane was immunoblotted with a rabbit anti-GAT-1. The boxed region represents the mature form of GAT-1 in cells. CHO stands for Chinese hamster ovary cells. CHO cells were used for control because of the low level of the endogenous GAT-1 expression. (C) The graph represents the normalized integrated protein density values (IDVs) of the mature form of GAT-1 defined by being Endo-H resistant normalized to the wildtype mature form of GAT-1 (bands 1 + 2). (D) The graph represents the normalized IDVs of the immature form of GAT-1 defined by being Endo-H unresistant (shifted to a lower level after H digestion) normalized to the wildtype immature form of GAT-1 (Band 3 shifted to Band 4). N = 4–5 different transfections, δδδ P < 0.001 overall mutations versus wt, *P < 0.05, **P < 0.01, ***P < 0.001 versus wt, one-way analysis of variance and Newman–Keuls test. Values were expressed as mean ± SEM.