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. 2022 Jun 6;4(3):fcac144. doi: 10.1093/braincomms/fcac144

Figure 7.

Figure 7

Both SLC6A1+/A288V and SLC6A1+/S295L mice had reduced GAT-1 protein that was partially restored by 4-phenylbutyrate acid (PBA). (A–H) Lysates from different brain regions [cortex (cor), cerebellum (cb), hippocampus (hip) and thalamus (thal)] from the wildtype (wt) and heterozygous (het) mice at 4–6 months old, untreated (A, B) or treated with vehicle or PBA (100 mg/kg) for 7 days (E, F) were subjected to SDS-PAGE and immunoblotted with anti-GAT-1 antibody. (C, D) Integrated density values (IDVs) for total GAT-1 from wildtype and het KI were normalized to the Na+/K+ ATPase or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) loading control (LC) in each specific brain region and plotted. N = 4 from four pairs of mice. (G, H) Integrated density values (IDVs) for total GAT-1 from het KI treated with vehicle or treated with PBA were normalized to the Na+/K+ ATPase or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) loading control (LC). The IDVs of the heterozygous treated with PBA were then normalized to vehicle treated. The vehicle treated in each brain region was taken as 1. N = 4 from four pairs of mice for C, D, G and H. Values were expressed as mean ± SEM. One-way analysis of variance or unpaired t test. In C and D, ***P < 0.001 versus wt, in G and H, **P < 0.01; ***P < 0.001 versus vehicle treated.